Simmons Citrate Agar- Composition, Principle, Preparation, Results, Uses

Result Interpretation on Simmons Citrate Agar

Simmons citrate agar is a selective and differential medium that tests for an organism’s ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. It is used for differentiating gram-negative bacteria on the basis of citrate utilization. It is useful for selecting organisms that use citrate as their main carbon and energy source. Initially, the citrate medium was developed by Koser containing ammonium salt as the only nitrogen source and citrate as the only carbon …Read more