Deoxycholate Citrate Agar (DCA)- Composition, Principle, Preparation, Results, Uses

Deoxycholate Citrate Agar (DCA)

Deoxycholate Citrate Agar is a modification of Leifson formula and is recommended for the isolation of Salmonella and Shigella spp. This medium is similar to deoxycholate agar in comparison but is moderately more selective for enteric pathogens owing to increased concentrations of both citrate and deoxycholate salts. Sodium deoxycholate at pH 7.3 to 7.5 is inhibitory for gram-positive bacteria. Citrate salts, in the concentration included in the formulation, are inhibitory to gram-positive bacteria and most other normal intestinal organisms. It …Read more