Lysine Iron Agar (LIA)- Composition, Principle, Preparation, Results, Uses
Lysine iron agar (LIA) is a differential medium used to test organisms for the ability to deaminate lysine or decarboxylate lysine. Lysine deamination is an aerobic process that occurs on the slant of the media. Lysine decarboxylation is an anaerobic process that occurs in the butt of the media. Edwards and Fife designed LIA in 1961 to presumptively identify Salmonella species, including lactose fermenting Salmonella arizonae, which has been implicated in foodborne outbreaks of gastroenteritis. Composition of Lysine Iron Agar (LIA) Ingredients Gms/liter Peptone 5.000 Yeast extract 3.000 Dextrose (Glucose) 1.000 L-Lysine 10.00 Ferric …Read more