Rambach Agar Preparation

The first chromogenic culture medium for the detection of E. coli was invented and patented by Dr. A. Rambach in 1979. This technology is a color-based differentiation method. It is based on soluble colorless molecules (called chromogens), composed of a substrate (targeting a specific enzymatic activity) and a chromophore. When the target organism’s enzyme cleaves the colorless chromogenic conjugate, the chromophore is released. In its unconjugated form, the chromophore exhibits its distinctive color and, due to reduced solubility, forms a …Read more