Silver Staining

Grocott-Gomori’s Methenamine Silver Staining

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Grocott-Gomori's Methenamine Silver Staining

Grocott-Gomori’s Methenamine Silver (GMS) stain is a histological stain that is used majorly for the identification of carbohydrates in fungal microorganisms. This staining method was named after György Gömöri, a physician from Hungary, who developed the staining methodology. Its initial application to assess missing tissues and diseases in the liver and the rectum (Nadworny, Wang, Tredget, & Robert, 2010) and then used for the identification of Pneumocystis jiroveci, a fungus known to cause an opportunistic infection called pneumocytosis, in immunocompromised and …Read more

Silver Staining- Principle, Procedure, Applications

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Silver Staining

What is silver staining? Silver staining is a special yet powerful staining technique that is used for the detection and identification of proteins in gels. This is because silver binds to the chemical terminal or side chains of amino groups i.e carboxyl and sulfhydryl groups. It has been used for decades now to separate proteins from polyacrylamide gel electrophoresis. The nucleation sites where there are tiny crevices where the free gas-liquid surface is maintained in proteins, promote formaldehyde reduction of …Read more