Sandwich ELISA- Steps and Advantages
The sandwich ELISA quantify antigens between two layers of antibodies (i.e. capture and detection antibody). Sandwich ELISA is named so as antigen is sandwiched between two antibodies. The sandwich assay uses two different antibodies that are reactive with different epitopes on the antigen with a concentration that needs to be determined. The antigen to be measured must contain at least two antigenic epitope capable of binding to antibody, since at least two antibodies act in the sandwich. Either monoclonal or …Read more