TCBS Agar

TCBS Agar- Composition, Principle, Preparation, Results, Uses

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Result Interpretation on Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar

Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar, is a type of selective agar that is used in microbiology laboratories to isolate Vibrio species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae and other Vibrio species from clinical specimens and other materials. TCBS Agar was developed by Kobayashi et al, who modified the selective medium of Nakanishi. Although this medium was originally designed for the isolation of V. cholerae and V. parahaemolyticus, most Vibrios grow to healthy large colonies with different colonial …Read more

为什么tcb琼脂不是热压处理过的?

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Why TCBS agar is not autoclaved

Answer: Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios. TCBS agar is composed of yeast extract, peptone, sodium thiosulfate, sodium citrate, ox bile (oxgall), sucrose, sodium chloride, iron (III) citrate, bromothymol blue, thymol blue, agar, and distilled or deionized water. TCBS is not autoclaved because: This media contains Ox bile (Oxgall), a derivative of bile salts which inhibit gram-positive bacteria might be sensitive while autoclaving. This agar needs to be boiled …Read more