Lysine Iron Agar (LIA)
Lysine iron agar (LIA) is a differential medium used to test organisms for the ability to deaminate lysine or decarboxylate lysine. Lysine deamination is an aerobic process which occurs on the slant of the media. Lysine decarboxylation is an anaerobic process which occurs in the butt of the media. Edwards and Fife designed LIA in 1961 to presumptively identify Salmonella species, including lactose fermenting Salmonella arizonae, which has been implicated in foodborne outbreaks of gastroenteritis. Composition of Lysine Iron Agar (LIA) Ingredients Gms/litre Peptone 5.000 Yeast extract 3.000 Dextrose (Glucose) 1.000 L-Lysine 10.00 Ferric ammonium …Read more